Live imaging of heart development
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As part of an international research network (see LiveHeart and 4DHeart), this new project aims at investigating zebrafish heart development and regeneration by devising new in vivo imaging strategies.
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Multicolor live imaging of the zebrafish beating heart using multiphoton light-sheet microscopy. The spatio-temporal resolution is sufficient to track individual cell movements during a cardiac cycle. Adapted from Mahou et al, 2014.
Related publications
Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency
V. Maioli, A. Boniface, P. Mahou, J. Ferrer Ortas, L. Abdeladim, E. Beaurepaire, W. Supatto
Biomedical Optics Express, 11(10), 6012-6026 (2020).
Multicolor two-photon light-sheet microsocopy.
Mahou P, Vermot J, Beaurepaire E, Supatto W
Nature Methods (2014)
Advances in whole-embryo imaging: a quantitative transition is underway.
Pantazis P, Supatto W
Nature Reviews Molecular Cell Biology (2014)