Ongoing advances in optical imaging open novel possibilities for studying intact biological tissues with subcellular resolution. We develop experimental strategies for removing bottlenecks in experimental systems biology based on our expertise in nonlinear optics, tissue microscopy, image analysis, cell/developmental biology, and biophysics. Our activity consists of two complementary aspects (i) microscopy methods development including proof-of-principle studies, and (ii) studying embryonic development and tissue function using multidisciplinary approaches.

Methods: biophotonics, multiphoton microscopy, light-sheet, 2PEF, 3PEF, SHG, THG, FLIM, CARS, SIM, polarization, laser ablation, terahertz. Topics: embryogenesis, neurodevelopment, collagen, heart, cornea, extracellular matrix. Models: zebrafish, drosophila, mouse (ex vivo), tissue biopsies, cell cultures. 

PEOPLE

Collaborators and Alumni • Publications • Work with us 

ONGOING PROJECTS
Advanced microscopies
• Second harmonic generation (SHG) in skin and cornea
• Multiphoton microscopy of developing tissues
   - Multicolor two-photon microscopy (wavelength mixing, brainbow)
   - Two-photon light sheet microscopy (2P-SPIM)
   - FLIM-based metabolic imaging
   - Deep-tissue 3-photon (3PEF) microscopy
   - Label-free third harmonic generation (THG) microscopy
   - Wavefront control (adaptive optics, Bessel beams)
• Polarimetric nonlinear microscopy
• Terahertz spectroscopy and imaging
• Large-volume bioimage informatics
• Super-resolution imaging (SIM)
• Equipex Morphoscope
Embryonic and organ development
• Cilia imaging and left-right symmetry breaking
• Live imaging of heart development & regeneration in zebrafish
• Cell migration during zebrafish embryo gastrulation
Biomaterials structure and function
• Transparency of the human cornea
• Multiphoton characterization of cultural heritage artefacts
...
Past projects
• Tissue micromanipulation and embryo biomechanics
• Nonlinear microscopy with broadband ultrashort pulses

06/2022 - Microscopy unit @LOB BBQ