Microscopies avancées & physiologie des tissus

Methods: biophotonics, multiphoton microscopy, light-sheet, 2PEF, 3PEF, SHG, THG, FLIM, CARS, SIM, polarization, laser ablation, terahertz. Topics: embryogenesis, neurodevelopment, collagen, heart, cornea, extracellular matrix. Models: zebrafish, drosophila, mouse (ex vivo), tissue biopsies, cell cultures.
PEOPLE
• Emmanuel Beaurepaire (CNRS DR)
• Anatole Chessel (EP Asst Prof)
• Nicolas David (CNRS CR)
• Guilhem Gallot (CNRS DR)
• Gaël Latour (UPSay Asst Prof)
• Pierre Mahou (EP IR)
• Karsten Plamann (ENSTA PR)
• Marie-Claire Schanne-Klein (CNRS DR)
• Chiara Stringari (CNRS CR)
• Willy Supatto (CNRS DR)
Postdocs
• Payvand Arjmand
• Bahar Asadipour
• Sophie Escot
• Florence Giger
• Matthew Gleeson
• Lei Zhu
• Xue Bing
• Hugo Blanc (PhD 11/2023)
• Nuno Bras
• Clément Caporal
• Stella Dees
• Amélie Elouin
• Giulia Galante
• Blandine Lordon
• Poncia Nyembo
• Solène Prudhomme
Technical support
• Xavier Solinas - electronics
• Jean-Marc Sintes - mechanics
• Isabelle Lamarre-Jouenne - biotechnology
• Emilie Menant - zebrafish husbandry
Collaborators and Alumni • Publications • Work with us
ONGOING PROJECTS
Advanced microscopies
• Second harmonic generation (SHG) in skin and cornea
• Multiphoton microscopy of developing tissues
- Multicolor two-photon microscopy (wavelength mixing, brainbow)
- Two-photon light sheet microscopy (2P-SPIM)
- FLIM-based metabolic imaging
- Deep-tissue 3-photon (3PEF) microscopy
- Label-free third harmonic generation (THG) microscopy
- Wavefront control (adaptive optics, Bessel beams)
• Polarimetric nonlinear microscopy
• Terahertz spectroscopy and imaging
• Large-volume bioimage informatics
• Super-resolution imaging (SIM)
• Equipex Morphoscope
Embryonic and organ development
• Cilia imaging and left-right symmetry breaking
• Live imaging of heart development & regeneration in zebrafish
• Cell migration during zebrafish embryo gastrulation
Biomaterials structure and function
• Transparency of the human cornea
• Multiphoton characterization of cultural heritage artefacts
...
Past projects
• Tissue micromanipulation and embryo biomechanics
• Nonlinear microscopy with broadband ultrashort pulses
06/2023 - Microscopy unit @picnic
06/2022 - Microscopy unit @LOB BBQ