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Molecular detail on the spatio-temporal regulation of Rac1 signalling

le 7 mars 2016 à 11 h
Amanda Remorino, Institut Curie, Paris

After a brief overview of my PhD work on 2D-IR spectroscopy, I will present results that reveal the spatio-temporal distribution of the microscopic parameters underlying Rac1 activation profiles at the cellular scale. The small RhoGTPase Rac1 is involved in the signaling network regulating cell polarization and migration. Rac1 is activated and deactivated at the plasma membrane through its interaction with GEFs and GAPs respectively. In its active form, Rac1 recruits a variety of effectors controlling the dynamics of the cell cytoskeleton and thus cell morphology. It has been shown that Rac1 presents detailed patterns of activation/deactivation with micrometer and millisecond resolutions key to the generation and maintenance of a polarized cell state. In this work, I have developed a methodology to interrogate the molecular architecture and dynamics of Rac1 as a function of its activation state by combining optogenetics, single molecule tracking, super-resolution imaging, and protein micro-pattering.  


Lieu(x) :         Amphithéâtre Curie, Ecole Polytechnique


Contact :       Cedric Bouzigues
                      cedric.bouzigues at polytechnique.edu