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Roxane Lestini English version

Maitre de Conférences / Assistant professor - Ecole polytechnique
Pièce : 84-20 12
Tél :+33 (0) 16933 5028
roxane.lestini at polytechnique.edu
       

 

Scientific Interests


Replication, Recombination and Repair (3Rs) in archaea using Haloferax volcanii as a model organism

 

Background


2014-present: MdC at Laboratory for Optics and Biosciences, CNRS / INSERM / Ecole Polytechnique, France

2012-2014: Chargé d'enseignement at Laboratory for Optics and Biosciences, CNRS / INSERM / Ecole Polytechnique, France

2009-2012: Post-doctoral position at Laboratory for Optics and Biosciences, CNRS / INSERM / Ecole Polytechnique, France

2008-2009: Post-doctoral position at Nottingham University, UK, in Dr. Thosten Allers laboratory

2004-2008: PhD on replication fork restart in E. coli at the CGM, CNRS, Gif-sur-yvette, supervised by Dr. Bénédicte Michel

 

Teaching


MODAL (Practical Course) for 2nd year student at Ecole polyetchnique untitled « Bioluminescence et GFP » 

TREX (Practical Course) for 3rd year student at Ecole polyetchnique untitled « Rôle des flagellines dans la motilité chez l’archée Haloferax volcanii » 

Master 2 Biologie Santé, Parcours Microbiologie Fondamentale – Paris XI/Paris Saclay « Une révolution récente en (micro)biologie : les archées »

 

Publications


Plateau, P., Saveanu, C., Lestini, R., Dauplais, M., Decourty, L., Jacquier, A., Blanquet, S. and Lazard, M. (2017) Exposure to selenomethionine causes selenocysteine misincorporation and protein aggregation in Saccharomyces cerevisiae. Sci Rep, 7, 44761

Becker, H.F., Heliou, A., Djaout, K., Lestini, R., Regnier, M. and Myllykallio, H. (2017) High-throughput sequencing reveals circular substrates for an archaeal RNA ligase. RNA Biol, 1-11.

Lestini, R., Delpech, F. and Myllykallio, H. (2015) DNA replication restart and cellular dynamics of Hef helicase/nuclease protein in Haloferax volcanii. Biochimie, 118, 254-263.

Lestini, R., Delpech, F. and Myllykallio, H. (2015) DNA Replication Restart in Archaea, Advances in DNA Repair, Prof. Clark Chen (Ed.), InTech, DOI: 10.5772/59994.

Rezgui, R., Lestini, R., Kuhn, J., Fave, X., McLeod, L., Myllykallio, H., Alexandrou, A. and Bouzigues, C. (2014) Differential interaction kinetics of a bipolar structure-specific endonuclease with DNA flaps revealed by single-molecule imaging. PLoS One, 9, e113493.

Lestini, R., Laptenok, S.P., Kuhn, J., Hink, M.A., Schanne-Klein, M.C., Liebl, U. and Myllykallio, H. (2013) Intracellular dynamics of archaeal FANCM homologue Hef in response to halted DNA replication. Nucleic Acids Res, 41, 10358-10370.

Creze, C., Ligabue, A., Laurent, S., Lestini, R., Laptenok, S.P., Khun, J., Vos, M.H., Czjzek, M., Myllykallio, H. and Flament, D. (2012) Modulation of the Pyrococcus abyssi NucS endonuclease activity by replication clamp at functional and structural levels. J Biol Chem, 287, 15648-15660.

Lestini, R., Creze, C., Flament, D., Myllykallio, H., and Henneke, G., (2011). Archaeal DNA Repair Nucleases, DNA Repair - On the Pathways to Fixing DNA Damage and Errors, Francesca Storici (Ed.), InTech, DOI: 10.5772/23361

Creze, C., Lestini, R., Kuhn, J., Ligabue, A., Becker, H.F., Czjzek, M., Flament, D. and Myllykallio, H. (2011) Structure and function of a novel endonuclease acting on branched DNA substrates. Biochem Soc Trans, 39, 145-149.

Lestini, R., Duan, Z. and Allers, T. (2010) The archaeal Xpf/Mus81/FANCM homolog Hef and the Holliday junction resolvase Hjc define alternative pathways that are essential for cell viability in Haloferax volcanii. DNA Repair (Amst), 9, 994-1002.

Baharoglu, Z.*, Lestini, R.*, Duigou, S. and Michel, B. (2010) RNA polymerase mutations that facilitate replication progression in the rep uvrD recF mutant lacking two accessory replicative helicases. Mol Microbiol, 77, 324-336 *co-premier auteur

Lestini, R. and Michel, B. (2008) UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR in a rep mutant of Escherichia coli. J Bacteriol, 190, 5995-6001.

Centore, R.C., Lestini, R. and Sandler, S.J. (2008) XthA (Exonuclease III) regulates loading of RecA onto DNA substrates in log phase Escherichia coli cells. Mol Microbiol, 67, 88-101.

Michel, B., Boubakri, H., Baharoglu, Z., LeMasson, M. and Lestini, R. (2007) Recombination proteins and rescue of arrested replication forks. DNA Repair (Amst), 6, 967-980.

Michel, B., Baharoglu, Z., and Lestini, R. (2007) Genetics of recombination in the model bacteriun Escherichia coli. Topics in Current Genetics, Aguilera A. and Rothstein R. eds, Springer.

Lestini, R. and Michel, B. (2007) UvrD controls the access of recombination proteins to blocked replication forks. EMBO J, 26, 3804-3814.

Janniere, L., Canceill, D., Suski, C., Kanga, S., Dalmais, B., Lestini, R., Monnier, A.F., Chapuis, J., Bolotin, A., Titok, M. et al. (2007) Genetic evidence for a link between glycolysis and DNA replication. PLoS One, 2, e447.