Publications

2006

• Observer les neurones grâce aux ondes térahertz
  
  • Gallot Guilhem
  La Recherche, Sciences et avenir, 2006, 397, pp.26. Des chercheurs de l'École polytechnique ont développé un dispositif d'imagerie des neurones, fondée sur l'utilisation d'ondes térahertz [1]. Ils observent ainsi les mouvements d'ions et d'eau à l'intérieur et à proximité des neurones.
• Micrometer scale ex vivo multiphoton imaging of unstained arterial wall structure
  
  • Boulesteix Thierry
  • Pena Ana-Maria
  • Pagès Nicole
  • Godeau G.
  • Sauviat Martin-Pierre
  • Beaurepaire Emmanuel
  • Schanne-Klein Marie-Claire
  Cytometry Part A, Wiley, 2006, 69A (1), pp.20-26. We characterize the application of multiphoton microscopy to the observation of the extracellular matrix of fresh unstained vessels. Combined two-photon-excited fluorescence (2PEF) and second harmonic generation (SHG) imaging of large arteries reveals the architecture of elastin and collagen fibers in the vessel wall with remarkable specificity. We present elastin/collagen imaging in unstained rat vessels at both micrometer and whole vessel scales, and we characterize the optical properties of rat carotid artery and aorta walls. We apply this method to evidence deleterious effects of residual doses of a pesticide on the vessel wall. This study illustrates the potential of 2PEF/SHG microscopy for pharmacological studies in unlabeled arteries. © 2005 Wiley-Liss, Inc. (10.1002/cyto.a.20196)
  DOI : 10.1002/cyto.a.20196
• Chiroptical effects in the second harmonic generation from collagens I and IV: applications in nonlinear microscopy
  
  • Pena Ana-Maria
  • Boulesteix Thierry
  • Dartigalongue Thibault
  • Strupler Mathias
  • Beaurepaire Emmanuel
  • Schanne-Klein Marie-Claire
  Nonlinear optics, quantum optics, Old City Pub., 2006, 35 (1-3), pp.235-240. An emerging application of multiphoton microscopy is the observation of unstained intact biological tissues based on intrinsic sources of nonlinear signals. However, reliable biomedical imaging requires a thorough analysis of these endogenous signals. Looking at skin biopsies, we focused on 2-Photon Excited Fluorescence (2PEF) arising from cytokeratins in the epidermis, and second harmonic generation (SHG) from collagen fibers in the dermis. We determined the 2PEF excitation spectrum and action cross section of purified keratins from human epidermis, and obtained a good agreement with in situ measurements. In order to analyze the role of chirality and collagen type in SHG signal, we performed polarization-resolved surface SHG experiments on thin films of collagens I and IV molecules. We observed that collagen I and IV molecules exhibit comparable SHG signals, except for the chiral contributions which are absent for collagen IV and represent typically 50% of the signal for collagen I. We concluded that the large collagen I SHG efficiency is dominated by coherent effects due to the high density and quasi-crystalline order in collagen fibrils, whereas the lack of any alignment within the collagen IV molecules explains the absence of signal from this collagen type in SHG microscopy.
• Functional implications of the propionate 7 Arginine 220 interaction in the FixLH oxygen sensor from Bradyrhizobium japonicum
  
  • Balland Véronique
  • Bouzhir-Sima Latifa
  • Anxolabéhère-Mallart E.
  • Boussac A.
  • Vos Marten H.
  • Liebl Ursula
  • Mattioli T.
  Biochemistry, American Chemical Society, 2006, 45, pp.2072-2084.
• Role of Heme Iron Coordination and Protein Structure in the Dynamics and Geminate Rebinding of Nitric Oxide to H93G Myoglobin : Implications for NO-Sensors
  
  • Négrerie Michel
  • Kruglik Sergei
  • Lambry Jean-Christophe
  • Vos Marten H.
  • Martin Jean-Louis
  • Franzen Stefan
  Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2006, 281, pp.10389-10398.
• Role of distal arginine in early sensing intermediates in the heme domain of the oxygen sensor FixL
  
  • Jasaitis Audrius
  • Hola Klara
  • Bouzhir-Sima Latifa
  • Lambry Jean-Christophe
  • Balland Véronique
  • Vos Marten H.
  • Liebl Ursula
  Biochemistry, American Chemical Society, 2006, 45, pp.6018-6026.
• Molécules d'origine naturelle et nouveaux médicaments anticancéreux [Molecules of natural origin and new anticancer drugs]
  
  • Pages N.
  • Goudey-Perriere Françoise
  • Sauviat Martin-Pierre
  • Benoit Evelyne
  • Chamorro G.
  , 2006, pp.89-98.
• Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity
  
  • Hunt D.E.
  • Klepac-Ceraj V.
  • Acinas S.G.
  • Bertilsson S.
  • Gautier Christian
  • Polz M.F.
  Applied and Environmental Microbiology, American Society for Microbiology, 2006, 72 (3), pp.2221. The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample. Copyright Cop. 2006, American Society for Microbiology. All Rights Reserved. (10.1128/AEM.72.3.2221-2225.2006)
  DOI : 10.1128/AEM.72.3.2221-2225.2006
• A filtering procedure for systematic removal of pump-perturbed polarization artifacts
  
  • Polack Thomas
  Optics Express, Optical Society of America - OSA Publishing, 2006, 14, pp.5823.
• Terahertz achromatic quarter-wave plate
  
  • Masson Jean-Baptiste
  • Gallot Guilhem
  Optics Letters, Optical Society of America - OSA Publishing, 2006, 31 (2), pp.265. Phase retardera usually present a strong frequency dependence. We discuss the design and characterization of a terahertz achromatic quarter-wave plate. This wave plate is made from six birefringent quartz plates precisely designed and stacked together. Phase retardation has been measured over the whole terahertz range by terahertz polarimetry. This achromatic wave plate demonstrates a huge frequency bandwidth (?max/?min ˜ 7), and therefore can be applied to terahertz time domain spectroscopy and polarimetry. Cop. 2006 Optical Society of America. (10.1364/OL.31.000265)
  DOI : 10.1364/OL.31.000265
• Photoinduced electron transfer from a novel nanotrigger addressed to the NADPH site within the endothelial NO-synthase to the flavin moieties of the protein
  
  • Beaumont Edward
  • Robin Anne-Claire
  • Berka Vladimir
  • Tsai Ah-Lim
  • Blanchard-Desce Mireille
  • Lambry Jean-Christophe
  • Slama-Schwok Anny
  Nitric Oxide: Biology and Chemistry, Elsevier, 2006, 14 (4), pp.14-15. We developed a new selective molecular tool to trigger enzymatic activity in a synchronous manner and monitor the sequence of the kinetic events by ultra-fast transient spectroscopy. Our approach is based on a synthetic nanotrigger addressing a selected site within proteins, namely the conserved NADPH binding site common to many enzymes involved in bioreductive processes [1]. The nanotrigger combines a "docking" subunit responsible for the recognition of NADPH sites within proteins and a "chromophoric" subunit responsive to light excitation and able to transfer electrons to the flavin moieties of proteins. We present the first spectroscopic data on such a nanotrigger [1] in the presence of the reductase domain of the endothelial nitric oxide synthase eNOSred. (10.1016/j.niox.2006.04.050)
  DOI : 10.1016/j.niox.2006.04.050
• Nouvelle approche des fibroses par microscopie multiphotonique avec génération de second harmonique [New approach of fibrosis by multiphoton microscopy with second harmonic generation]
  
  • Hernest Monica
  • Pena Ana-Maria
  • Strupler Mathias
  • Beaurepaire Emmanuel
  • Martin Jean-Louis
  • Schanne-Klein Marie-Claire
  • Tharaux Pierre-Louis
  Médecine/Sciences, EDP Sciences, 2006, 22 (10), pp.820-821. La fibrose est une réponse adaptative pathologique qui détruit non spécifiquement les tissus. Il s'agit d'un processus universel de réparation des tissus qui survient en réaction à de nombreux types d'agressions telles les contraintes mécaniques, les brûlures, les radiations ionisantes, l'ischémie, l'inflammation. Ces agressions concourent de manière intriquée à la physiopathologie des maladies infectieuses, tumorales ou auto-immunes, de l'hypertension artérielle et des maladies cardio-vasculaires. Le terme de fibrose décrit précisément l'accumulation nouvelle de protéines de la matrice extra-cellulaire selon un arrangement spatial fibrillaire caractéristique. Il s'agit essentiellement de molécules de collagènes de type I et III (voire de type II, V ou XI) synthétisées sous forme de triples hélices elles-mêmes assemblées en fibrilles par les cellules fibroblastiques. L'apparition des collagènes fibrillaires marque un changement qualitatif et quantitatif de composition des collagènes des tissus. Réciproquement, ces changements de la matrice extracellulaire influencent le phénotype des cellules qui y résident. Ainsi dans le rein normal, le collagène de type I n'existe que dans l'adventice artériel. Son apparition au sein des autres structures de cet organe marque une fibrose tubulo-interstitielle qui constitue le meilleur marqueur pronostic défavorable d'une évolution vers l'insuffisance rénale terminale, et ce quelle que soit la maladie causale. Ainsi, comme lors des fibroses compliquant les hépatopathies et les pneumopathies chroniques, les séquelles de brûlures ou d'abrasions cutanées-muqueuses ou encore le remodelage cardiaque et vasculaire, le réarrangement de la géométrie de la matrice extracellulaire altère l'organisation fonctionnelle du tissu considéré. De ce fait, ce processus de réparation a des effets fonctionnels délétères qui constituent un enjeu médical majeur. Les fibrilles de collagène ont des capacités d'auto-assemblage qui sont aussi catalysées et stabilisées ou au contraire empêchées par les enzymes de la matrice extracellulaire. Le développement de la fibrose ou sa régression dépend donc ainsi du bilan des équilibres biologiques de ces mécanismes. Il est donc crucial de caractériser les changements extracellulaires et cellulaires qui font du restutio ad integrum de l'architecture et de la fonction tissulaire un défi biomédical. (10.1051/medsci/20062210820)
  DOI : 10.1051/medsci/20062210820
• Nonlinear microscopy using Second Harmonic Generation from myosin filaments and fibrillar collagens
  
  • Pena Ana-Maria
  • Strupler Mathias
  • Boulesteix Thierry
  • Fabre Aude
  • Hernest Monica
  • Tharaux Pierre-Louis
  • Crestani Bruno
  • Martin Jean-Louis
  • Sauviat Martin-Pierre
  • Beaurepaire Emmanuel
  • Schanne-Klein Marie-Claire
  , 2006.
• Mechanisms involved in the swelling of erythrocytes caused by Pacific and Caribbean ciguatoxins
  
  • Sauviat Martin-Pierre
  • Boydron-Le Garrec Raphaële
  • Masson Jean-Baptiste
  • Lewis Richard L.
  • Vernoux Jean-Paul
  • Molgó Jordi
  • Laurent Dominique
  • Benoit Evelyne
  Blood Cells, Molecules and Diseases, Elsevier, 2006, 36(1), pp.1-9. The mechanisms underlying the swelling of frog red blood cells (RBC), induced by Pacific (P-CTX-1) and Caribbean (C-CTX-1) ciguatoxins (CTXs), were investigated by measuring the length, width and surface of their elliptic shape. P-CTX-1 (0.5 to 5 nM) and C-CTX-1 (1 nM) induced RBC swelling within 60 min. The CTXs-induced RBC swelling was blocked by apamin (1 μM) and by Sr2+ (1 mM). P-CTX-1-induced RBC swelling was prevented and inhibited by H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (27 μM), an inhibitor of soluble guanylate cyclase (sGC), and NOS blockade by NG methyl-l-arginine (l-NMA; 10 μM). Cytochalasin D (cytD, 10 μM) increased RBC surface and mimicked CTX effect but did not prevent the P-CTX-1-induced l-NMA-sensitive extra increase. Calculations revealed that P-CTX-1 and cytD increase RBC total surface envelop and volume. These data strongly suggest that the molecular mechanisms underlying CTXs-induced RBC swelling involve the NO pathway by an activation of the inducible NOS, leading to sGC activation which modulates intracellular cGMP and regulates L-type Ca2+ channels. The resulting increase in intracellular Ca2+ content, in turn, disrupts the actin cytoskeleton, which causes a water influx and triggers a Ca2+-activated K+ current through SK2 isoform channels. (10.1016/j.bcmd.2005.10.007)
  DOI : 10.1016/j.bcmd.2005.10.007
• Role of the middle residue in the triple tryptophan electron transfer chain of DNA photolyase: ultrafast spectroscopy of a Trp→Phe mutant
  
  • Lukacs Andras
  • Eker A.P.M.
  • Byrdin M.
  • Villette Sandrine
  • Pan J.
  • Brettel K.
  • Vos Marten H.
  Journal of Physical Chemistry B, American Chemical Society, 2006, 110 (32), pp.15654-15658.
• Endothelial nitric oxide synthase reduces nitrite anions to NO under anoxia
  
  • Gautier Clément
  • van Faassen E.
  • Mikula I.
  • Martasek P.
  • Slama-Schwok Anny
  Biochemical and Biophysical Research Communications, Elsevier, 2006, 341 (3), pp.816-821. In this work, we demonstrate that endothelial nitric oxide synthase is capable of anoxic reduction of nitrite anions to nitric oxide at physiological pH by absorption and EPR spectroscopy and electrochemical measurements. The nitrite reduction is achieved at the oxygenase domain of the protein and proceeds even in the absence of the tetrahydrobiopterin cofactor. The nitrite pathway increases by sixfold the NO production with respect to the regular arginine pathway under hypoxia, which is largely blocked. Therefore, basal levels of NO release could be sustained by anoxic nitrite reduction. The reaction suggests a new pathway for fast NO delivery under hypoxia, precisely when the vasodilating properties of nitric oxide are most needed. (c) 2006 Elsevier Inc. All rights reserved. (10.1016/j.bbrc.2006.01.031)
  DOI : 10.1016/j.bbrc.2006.01.031
• Single Lanthanide-doped Oxide Nanoparticles as Donors in Fluorescence Resonance Energy Transfer Experiments
  
  • Casanova Didier
  • Giaume D.
  • Gacoin Thierry
  • Boilot Jean-Pierre
  • Alexandrou Antigoni
  Journal of Physical Chemistry B, American Chemical Society, 2006, 110(39), pp.19264-19270.
• Comparative ultrafast dynamics of heme-ligand interactions in proteins
  
  • Vos Marten H.
  , 2006, pp.oral.
• Apport des toxines à la chimiothérapie anticancéreuse cytotoxique.
  
  • Pages N.
  • Goudey-Perriere Françoise
  • Sauviat Martin-Pierre
  • Benoit Evelyne
  • Chamorro G.
  , 2006, pp.81-88.
• Coupling between surface plasmons in subwavelength hole arrays
  
  • Masson Jean-Baptiste
  • Gallot Guilhem
  Physical Review B: Condensed Matter and Materials Physics (1998-2015), American Physical Society, 2006, 73, pp.121401.
• Manipulation of morphogenetic movements in live Drosophila embryos using femtosecond pulses
  
  • Supatto Willy
  • Débarre Delphine
  • Martin Jean-Louis
  • Schanne-Klein Marie-Claire
  • Farge Emmanuel
  • Beaurepaire Emmanuel
  , 2006.
• Ionic contrast terahertz near field imaging of axonal water fluxes
  
  • Masson Jean-Baptiste
  • Sauviat Martin-Pierre
  • Martin Jean-Louis
  • Gallot Guilhem
  Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2006, 103 (13), pp.4808-4812.
• Dynamique interne dans les hémoprotéines vue par spectroscopie ultrarapide
  
  • Vos Marten H.
  , 2006.
• Studying intra-protein signaling intermediates in the heme-based oxygen sensor protein FixL using sub-picosecond transient Raman spectroscopy
  
  • Vos Marten H.
  , 2006, pp.oral.
• Second harmonic generation by collagens I and IV: chiroptical and structural effects
  
  • Pena Ana-Maria
  • Boulesteix Thierry
  • Dartigalongue Thibault
  • Schanne-Klein Marie-Claire
  , 2006.