Chiara Stringari
Research scientist - CNRS
Room: 84-2017, 05-2016A
Phone: (+33) 1 69 33 5021
chiara.stringari at polytechnique.edu
Current research activity:
Advanced microscopies & tissue physiology
- FLIM-based metabolic imaging
- Third harmonic generation (THG) microscopy
Background
2014-present: Research scientist (CR1 CNRS), Lab. for optics and biosciences, Ecole Polytechnique, Palaiseau.
2013-2014: Postdoc, Lab. for optics and biosciences, Ecole Polytechnique, Palaiseau. (Dr. E. Beaurepaire)
2009-2013: Postdoctoral Fellow - Core Group Leader Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine. (Dr. E. Gratton)
2005-2008 PhD, Biophysics and Biophotonics Lab, European Laboratory for Non-Linear Spectroscopy (Dr. F.S. Pavone)
Publications
Selected publications:
Label-free imaging of red blood cells and oxygenation with color third-order sum-frequency generation microscopy
J. Ferrer Ortas, P. Mahou, S. Escot, C. Stringari, N. B. David, L. Bally-Cuif, N. Dray, M. Négrerie, W. Supatto, E. Beaurepaire
Light: Science & Applications (2023)
Coordinated metabolic transitions and gene expression by NAD+ during adipogenesis. E Sánchez-Ramírez, TPL Ung, A Alarcón del Carmen, X del Toro-Ríos, GR Fajardo-Orduña, LG Noriega, VA Cortés-Morales, AR Tovar, JJ Montesinos, R Orozco-Solís, C Stringari, L Aguilar-Arnal. J. Cell. Biol. 221 (12), e202111137 (2022)
Simultaneous NAD(P)H and FAD fluorescence lifetime microscopy of long UVA–induced metabolic stress in reconstructed human skin. T.P.L. Ung, S. Lim, X. Solinas, P. Mahou, A. Chessel, C. Marionnet, T. Bornschlögl, E. Beaurepaire, F. Bernerd, A.-M. Pena, C. Stringari. Sci. Rep. 11, 22171 (2021)
Modeling nonlinear microscopy near index-mismatched interfaces. J. Morizet, G. Sartorello, N. Dray, C. Stringari, E. Beaurepaire, N. Olivier. Optica 8 (7), 944-951 (2021)
High-speed polarization-resolved third-harmonic microscopy. J. Morizet, G. Ducourthial, W. Supatto, A. Boutillon, R. Legouis, M.-C. Schanne-Klein, C. Stringari, E. Beaurepaire. Optica 6 (3), 385-388 (2019)
Multicolor two-photon imaging of endogenous fluorophores in living tissues by wavelength mixing Stringari C., Abdeladim L., Malkinson G., Mahou P., Solinas X., Lamarre I., Brizion S., Galey J.B, Supatto W., Legouis R., Pena A.M., Beaurepaire E. Sci. Rep. 7(1):3792. (2017)
Spatial dynamics of SIRT1 dictate metabolic transitions in the cell nucleus. Aguilar-Arnal L.*, Ranjit S.*, Stringari C., Orozco-Solis R., Gratton E., Sassone-Corsi P. PNAS (2016)
Metabolic changes associated with methionine stress sensitivity in MDA-MB-468 breast cancer cells. Borrego S., Fahrmann J., Datta. R., Stringari C., Grapov D., Zeller M. Baldi P., Gratton. E., Keiser P. Cancer & Metabolism 2;4:9 (2016)
NADH fluorescence lifetime is an endogenous reporter of alpha-synuclein aggregation in live cells, Plotegher N., Stringari C, Jahid S., Veronesi M. , Girotto S., Gratton E., Bubacco L. FASEB J. 29(6):2484-94 (2015).
In Vivo single-cell detection of metabolic oscillations in stem cells. Stringari C, Wang H, Geyfman M, Crosignani V, Kumar V, Takahashi JS, Andersen B, Gratton E. Cell Rep. 10(1):1-7 (2015).
Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer. Pate K.T., Stringari C., Sprowl-Tanio S., Wang K., TeSlaa T., Hoverter N.P., McQuade M.M., Garner C., Digman M.A., Teitell M.A., Edwards R.A., Gratton E., Waterman M.L. EMBO J. 33(13):1454-73 (2014)
Two-photon excited fluorescence life-time, spectroscopy and imaging of melanins in-vitro and in-vivo. Krasieva T.B., Stringari C., Liu F., Sun C.H., Kong Y., Balu M., Meyskens F.L., Gratton E., and Tromberg B.J. J Biomed Opt.18(3):31107 (2013)
Metabolic trajectory associated with cellular proliferation in small intestine by Phasor Fluorescence Lifetime Microscopy of NADH, Stringari C., Edwards RA, Pate KT, Waterman ML, Donovan PJ, Gratton E. Sci. Rep. 2:568 (2012)
Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue. Stringari C., Cinquin A., Cinquin O., Digman MA., Donovan P., Gratton E. PNAS 108(33):13582-13587 (2011)
Probing myosin structural conformation in vivo by second-harmonic generation microscopy Nucciotti* V., Stringari* C., Sacconi* L., Vanzi* F., Fusi L., Linari M., Piazzesi G., Lombardi V. Pavone F.S. PNAS 107(17):7763-8 (2010)