SIFT: Label-Free Sorting by Droplet Microfluidics
November 25, 2021, 11AM
Paul ABBYAD, Santa Clara University, USA
Lieu(x) : Amphithéâtre Pierre Curie, Ecole Polytechnique
Contact : Antigoni Alexandrou
antigoni.alexandrou at polytechnique.edu
Our lab has developed a novel platform for droplet sorting that can be used to sort enzymes by activity, and cells by metabolism. It is a passive technique that utilizes no excitation laser, detector, active sorter and related electronics, or fluorescent labels. It presents a robust way to sort enzyme libraries for screening and sort cancer cells by a defining characteristic, their aberrant metabolism. The technique also enables the isolation of activated T-cells prior to the display of cellular surface markers. The isolation of activated T-cells in minutes rather than hours can have broad usage as a biotechnology tool and for the study and selection for immunology and immunotherapy.
Dobson, C; Zielke, C; Pan, C; Feit, C; Abbyad, P (2020) Method for Passive Droplet Sorting after Photo-Tagging Micromachines 11(11), 964. https://doi.org/10.3390/mi11110964
Zielke, C; Pan, C Gutierrez Ramirez, A; Feit, C; Dobson, C; Davidson, C; Sandel, B; Abbyad, P (2020) Microfluidic Platform for the Isolation of Cancer-Cell Subpopulations based on Single-Cell Glycolysis Analytical Chemistry 92, 10, 6949–6957. https://doi.org/10.1021/acs.analchem.9b05738
Horvath, DG; Braza, S; Moore, T; Pan, CW; Zhuc, L; Pak, O; Abbyad, P (2019) Sorting by interfacial tension (SIFT): Label-free enzyme sorting using droplet microfluidics Analytica Chimica Acta, 1089, 108-114. DOI: 10.1016/j.aca.2019.08.025
Pan, CW; Horvath, DG; Braza, S; Moore, T; Lynch, A; Feit C; Abbyad P (2019) Sorting by interfacial tension (SIFT): label-free selection of live cells based on single-cell metabolism Lab on a Chip, 19, 1344-1351 Front Cover of Journal; DOI: 10.1039/C8LC01328D
A télécharger: Séminaire Abbyad