Roxane Lestini
Pièce : 84-20 12
Tél :+33 (0) 16933 5028
roxane.lestini @polytechnique.edu
ORCID ID: 0000-0001-7377-4734
Google Scholar Account
Thèmes de recherche / Scientific Interests
Replication, Recombination and Repair (3Rs) in archaea using Haloferax volcanii as a model organism:
- In vivo localization of the replication proteins (e.g. Hef, RPA2) to understand replication dynamics
- Unraveling replication restart pathways by the identification and characterization of proteins involved
2008-2009: Post-doctoral position at Nottingham University, UK, in Dr. Thosten Allers laboratory
Publications (ORCID ID: 0000-0001-7377-4734)
Sodolescu A., Dian C., Terradot L., Bouzhir-Sima L., Lestini R., Myllykallio H., Skouloubris S., Liebl U. (2018). Structural and functional insight into serine hydroxymethyltransferase from Helicobacter pylori. PloS one, 13(12), e0208850. DOI
Delpech F., Collien Y., Mahou P., Beaurepaire E., Myllykallio M., Lestini R. Snapshots of archaeal DNA replication and repair in living cells using super-resolution imaging, Nucleic Acids Research, DOI
van Tran N., Muller L., Ross R. L., Lestini R., Létoquart J., Ulryck N., Limbach P. A., de Crécy-Lagard V., Cianférani S., Graille M. (2018). Evolutionary insights into Trm112-methyltransferase holoenzymes involved in translation between archaea and eukaryotes. Nucleic acids research, 46(16), 8483-8499, DOI
Sakina K., Lestini R., Myllykallio H. and Houali K. Isolation and identifcation of two extremely halophilic archaea from sebkhas in the Algerian Sahara (2018) Cellular and molecular biology 64(4):83-91
Plateau, P., Saveanu, C., Lestini, R., Dauplais, M., Decourty, L., Jacquier, A., Blanquet, S. and Lazard, M. (2017) Exposure to selenomethionine causes selenocysteine misincorporation and protein aggregation in Saccharomyces cerevisiae. Sci Rep, 7, 44761, DOI
Becker, H.F., Heliou, A., Djaout, K., Lestini, R., Regnier, M. and Myllykallio, H. (2017) High-throughput sequencing reveals circular substrates for an archaeal RNA ligase. RNA Biol, 1-11. DOI
Lestini, R., Delpech, F. and Myllykallio, H. (2015) DNA replication restart and cellular dynamics of Hef helicase/nuclease protein in Haloferax volcanii. Biochimie, 118, 254-263. DOI
Lestini, R., Delpech, F. and Myllykallio, H. (2015) DNA Replication Restart in Archaea, Advances in DNA Repair, Prof. Clark Chen (Ed.), InTech, DOI
Rezgui, R., Lestini, R., Kuhn, J., Fave, X., McLeod, L., Myllykallio, H., Alexandrou, A. and Bouzigues, C. (2014) Differential interaction kinetics of a bipolar structure-specific endonuclease with DNA flaps revealed by single-molecule imaging. PLoS One, 9, e113493. DOI
Lestini, R., Laptenok, S.P., Kuhn, J., Hink, M.A., Schanne-Klein, M.C., Liebl, U. and Myllykallio, H. (2013) Intracellular dynamics of archaeal FANCM homologue Hef in response to halted DNA replication. Nucleic Acids Res, 41, 10358-10370. DOI
Creze, C., Ligabue, A., Laurent, S., Lestini, R., Laptenok, S.P., Khun, J., Vos, M.H., Czjzek, M., Myllykallio, H. and Flament, D. (2012) Modulation of the Pyrococcus abyssi NucS endonuclease activity by replication clamp at functional and structural levels. J Biol Chem, 287, 15648-15660. DOI
Lestini, R., Creze, C., Flament, D., Myllykallio, H., and Henneke, G., (2011). Archaeal DNA Repair Nucleases, DNA Repair - On the Pathways to Fixing DNA Damage and Errors, Francesca Storici (Ed.), InTech, DOI
Creze, C., Lestini, R., Kuhn, J., Ligabue, A., Becker, H.F., Czjzek, M., Flament, D. and Myllykallio, H. (2011) Structure and function of a novel endonuclease acting on branched DNA substrates. Biochem Soc Trans, 39, 145-149. DOI
Lestini, R., Duan, Z. and Allers, T. (2010) The archaeal Xpf/Mus81/FANCM homolog Hef and the Holliday junction resolvase Hjc define alternative pathways that are essential for cell viability in Haloferax volcanii. DNA Repair (Amst), 9, 994-1002. DOI
Baharoglu, Z.*, Lestini, R.*, Duigou, S. and Michel, B. (2010) RNA polymerase mutations that facilitate replication progression in the rep uvrD recF mutant lacking two accessory replicative helicases. Mol Microbiol, 77, 324-336 *co-premier auteur DOI
Lestini, R. and Michel, B. (2008) UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR in a rep mutant of Escherichia coli. J Bacteriol, 190, 5995-6001. DOI
Centore, R.C., Lestini, R. and Sandler, S.J. (2008) XthA (Exonuclease III) regulates loading of RecA onto DNA substrates in log phase Escherichia coli cells. Mol Microbiol, 67, 88-101. DOI
Michel, B., Boubakri, H., Baharoglu, Z., LeMasson, M. and Lestini, R. (2007) Recombination proteins and rescue of arrested replication forks. DNA Repair (Amst), 6, 967-980. DOI
Michel, B., Baharoglu, Z., and Lestini, R. (2007) Genetics of recombination in the model bacteriun Escherichia coli. Topics in Current Genetics, Aguilera A. and Rothstein R. eds, Springer.
Lestini, R. and Michel, B. (2007) UvrD controls the access of recombination proteins to blocked replication forks. EMBO J, 26, 3804-3814. DOI
Janniere, L., Canceill, D., Suski, C., Kanga, S., Dalmais, B., Lestini, R., Monnier, A.F., Chapuis, J., Bolotin, A., Titok, M. et al. (2007) Genetic evidence for a link between glycolysis and DNA replication. PLoS One, 2, e447. DOI
Bidnenko V., Lestini R., Michel B.The Escherichia coli UvrD helicase is essential for Tus removal during recombination-dependent replication restart from Ter sites.Mol Microbiol. 2006 Oct;62(2):382-96.DOI