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Multiphoton characterization of cultural heritage artefacts


Staff

Marie-Claire Schanne-Klein / DR CNRS
M. Schmeltz / PhD student

Close collaboration with Gaël Latour / Univ Paris-Sud


Topic

Multiphoton microscopy is a valuable tool to characterize heritage artefacts that are heterogeneous scattering media like biological tissues.We characterized the multilayer structure of varnishes, and we probed the degradation of parchments, which are obtained from dried skin. We recently applied this new methodology to the Mappa Mundi d’Albi, which is classified as “World memory” by Unesco.


Figure: Mapping of a 17th century parchment using multiphoton microscopy. Well preserved regions (center) show SHG signals from collagen and slight fluorescence signals from fat or proteic residus, while altered regions only exhibit un-structured fluorescence signals because of collagen denaturation (so-called gelatinisation).


Highlights / review articles

[French] Cartographier la dégradation des parchemins à l’échelle microscopique sans contact ni prélèvement, Actualités scientifiques de l’Université Paris-Sud et du CNRS


Main ongoing collaborations

- G. Latour, IMNC (CNRS, Université Paris-Sud, Orsay)
- L. Robinet, CRC (CNRS, Museum National d’Histoire Naturelle, Paris)
- A. Dazzi, A. Deniset-Besseau, LCP (CNRS, Université Paris-Sud, Orsay)


Publications

Correlative nonlinear optical microscopy and infrared nanoscopy reveals collagen degradation in altered parchments, G. Latour, L. Robinet, A. Dazzi, F. Portier, A. Deniset-Besseau, and M.-C. Schanne-Klein, Sci. Rep. 6, 26344 (2016).

In situ 3D characterization of historical coatings and wood using multimodal nonlinear optical microscopy, G. Latour, J.-P. Echard, M. Didier, M.-C. Schanne-Klein, Opt. Express 20, 24623 (2012) pdf