En poursuivant votre navigation, vous acceptez l'utilisation de cookies destinés à améliorer la performance de ce site et à vous proposer des services et contenus personnalisés.

X

Multicolor / multimodal NLO microscopy


Experimental systems biology require novel investigation methods for imaging multiple physiological and structural parameters in intact biological tissues or embryos.

Multicolor two-photon imaging: we introduced a novel approach for simultaneous two-photon imaging of several fluorescent proteins (e.g. CFP, YFP, RFP) in tissues. This method, which is based on nonlinear wavelength mixing, is particularly appropriate for 'brainbow' tissue imaging, which we develop in collaboration with J. Livet's group at Institut de la Vision. By combining this method with automated serial tomography, we extended it to brain-wide multicolor imaging with micrometric resolution (ChroMS, chromatic multiphoton serial microscopy).

Multimodal imaging:
we develop approaches simultaneously providing complementary information such as fluorescence (2PEF), harmonic generation (SHG, THG), four-wave mixing (FWM, CARS), fluorescence lifetime (FLIM).


Related publications

Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy
L. Abdeladim, K.S. Matho, S. Clavreul, P. Mahou, J.-M. Sintes, X. Solinas, I. Arganda-Carreras, S.G. Turney, J.W. Lichtman, A. Chessel, A.-P. Bemelmans, K. Loulier, W. Supatto, J. Livet, E. Beaurepaire
Nature Communications 10, 1662 (2019)
Metrology of multiphoton microscopes using second harmonic generation nanoprobes
P. Mahou, G. Malkinson, E. Chaudan, T. Gacoin, E. Beaurepaire, W. Supatto
Small 13, 1701442 (2017).
Life-long neurogenic activity of individual neural stem cells and continuous growth establish an outside-in architecture in the teleost pallium
G. Furlan, V. Cuccioli, N. Vuillemin, L. Dirian, A. Janue Muntasell, M. Coolen, N. Dray, S. Bedu, C. Houart, E. Beaurepaire, I. Foucher, L. Bally-Cuif
Current Biology 27, 3288-3301 (2017).
Large-scale live imaging of adult neural stem cells in their endogenous niche
N. Dray, S. Bedu, N. Vuillemin, A. Alunni, M. Coolen, M. Krecsmarik, W. Supatto, E. Beaurepaire, and L. Bally-Cuif
Development 142: 3592-3600 (2015).
Multicolor two-photon light-sheet microscopy
P. Mahou, J. vermot, E. Beaurepaire, W. Supatto
Nature Methods 11(6), 600-601 (2014).
Multiplex cell and lineage tracking with combinatorial labels
K. Loulier, R. Barry, P. Mahou, Y. Le Franc, W. Supatto, K.S. Matho, S. Ieng, S. Fouquet, E. Dupin, R. Benosman, A. Chédotal, E. Beaurepaire, X. Morin, J. Livet
Neuron 81(3), 505-520 (2014).
Multicolor two-photon tissue imaging by wavelength mixing
P. Mahou, M. Zimmerley, K. Loulier, K. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre & E. Beaurepaire
Nature Methods 9(8) 815-818 (2012).
Combined third-harmonic generation and four-wave mixing microscopy of tissues and embryos
P. Mahou, N. Olivier, G. Labroille, L. Duloquin, J-M. Sintes, N. Peyriéras, R. Legouis, D. Débarre, and E. Beaurepaire
Biomed. Opt. Express 2(10), 2837-2849 (2011).
Cell lineage reconstruction of early zebrafish embryos using label-free nonlinear microscopy
N. Olivier, M. Luengo-Oroz, L. Duloquin, E. Faure, T. Savy, I. Veilleux, X. Solinas, D. Débarre, P. Bourgine, A. Santos, N. Peyriéras & E. Beaurepaire
Science 339(5994), 967-71 (2010).
Multimodal nonlinear imaging of the human cornea
F. Aptel, N. Olivier, A. Deniset-Besseau, J.-M. Legeais, K. Plamann, M.-C. Schanne-Klein & E. Beaurepaire
Invest. Ophtalmol. Vis. Sci. 51(5), 2459-65 (2010). Cover.
Harmonic microscopy of isotropic and anisotropic microstructure of the human cornea
N. Olivier, F. Aptel, K. Plamann, M.-C. Schanne-Klein & E. Beaurepaire
Opt. Express 18(5), 5028-40 (2010).