Fluorescence Lifetime Microscopy (FLIM)-based metabolic imaging
Contact: Chiara Stringari
Fluorescence Lifetime Microscopy (FLIM) of intrinsic biomarkers has demonstrated an exceptional capability to non-invasively investigate the energetic and metabolic redox state of living tissues at the cellular level.
We develop novel approaches for fast Fluorescence Lifetime Microscopy and novel label-free optical methods for metabolic imaging in stem cells and living tissues.
Multicolor two-photon imaging of endogenous fluorophores in living tissues by wavelength mixing C. Stringari, L. Abdeladim, G. Malkinson, P. Mahou, X. Solinas, I. Lamarre, S. Brizion, J.-B. Galey, W. Supatto, R. Legouis, A.-M. Pena, E. Beaurepaire
Scientific Reports 7, 3792 (2017).
In Vivo single-cell detection of metabolic oscillations in stem cells Stringari C, Wang H, Geyfman M, Crosignani V, Kumar V, Takahashi JS, Andersen B, Gratton E. Cell Rep. 10(1):1-7 (2015).
Metabolic trajectory associated with cellular proliferation in small intestine by Phasor Fluorescence Lifetime Microscopy of NADH Stringari C., Edwards RA, Pate KT, Waterman ML, Donovan PJ, Gratton E., Scientific Reports.2:568 (2012).
Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue. Stringari C., Cinquin A., Cinquin O., Digman MA., Donovan P., Gratton E. Proceedings of the National Academy of Sciences U S A 108(33):13582-13587 (2011)