Fluorescence Lifetime Microscopy (FLIM)-based metabolic imaging

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Fluorescence Lifetime Microscopy (FLIM) of intrinsic biomarkers has demonstrated an exceptional capability to non-invasively investigate the energetic and metabolic redox state of living tissues at the cellular level.

We develop novel approaches for fast Fluorescence Lifetime Microscopy and novel label-free optical methods for metabolic imaging in stem cells and living tissues.

Related publications

Coordinated metabolic transitions and gene expression by NAD+ during adipogenesis
E. Sánchez-Ramírez, T.P.L. Ung, A. Alarcón del Carmen, X. del Toro-Ríos, G.R. Fajardo-Orduña, L.G. Noriega, V.A. Cortés-Morales, A.R. Tovar, J.J. Montesinos, R. Orozco-Solís, C. Stringari, L. Aguilar-Arnal
J Cell Biol 221(12), e202111137 (2022)

Simultaneous NAD(P)H and FAD fluorescence lifetime microscopy of long UVA–induced metabolic stress in reconstructed human skin
T.P.L. Ung, S. Lim, X. Solinas, P. Mahou, A. Chessel, C. Marionnet, T. Bornschlögl, E. Beaurepaire, F. Bernerd, A.-M. Pena, C. Stringari
Scientific Reports 11, 22171 (2021)

Multicolor two-photon imaging of endogenous fluorophores in living tissues by wavelength mixing C. Stringari, L. Abdeladim, G. Malkinson, P. Mahou, X. Solinas, I. Lamarre, S. Brizion, J.-B. Galey, W. Supatto, R. Legouis, A.-M. Pena, E. Beaurepaire
Scientific Reports 7, 3792 (2017).

In Vivo single-cell detection of metabolic oscillations in stem cells Stringari C, Wang H, Geyfman M, Crosignani V, Kumar V, Takahashi JS, Andersen B, Gratton E. Cell Rep. 10(1):1-7 (2015).

Metabolic trajectory associated with cellular proliferation in small intestine by Phasor Fluorescence Lifetime Microscopy of NADH Stringari C., Edwards RA, Pate KT, Waterman ML, Donovan PJ, Gratton E., Scientific Reports 2:568 (2012).

Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue. Stringari C., Cinquin A., Cinquin O., Digman MA., Donovan P., Gratton E. Proceedings of the National Academy of Sciences U S A 108(33):13582-13587 (2011)