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Roxane Lestini

Assistant Professor - Ecole polytechnique
Pièce : 84-20 12
Tél :+33 (0) 16933 5028
roxane.lestini @polytechnique.edu

ORCID ID: 0000-0001-7377-4734
Google Scholar Account

       

 

Thèmes de recherche / Scientific Interests


Replication, Recombination and Repair (3Rs) in archaea using Haloferax volcanii as a model organism:

- In vivo localization of the replication proteins (e.g. Hef, RPA2) to understand replication dynamics

- Unraveling replication restart pathways by the identification and characterization of proteins involved

 

Background


2014-present: MdC at Laboratory for Optics and Biosciences, CNRS / INSERM / Ecole Polytechnique, France

2012-2014: Chargé d'enseignement at Laboratory for Optics and Biosciences, CNRS / INSERM / Ecole Polytechnique, France

2009-2012: Post-doctoral position at Laboratory for Optics and Biosciences, CNRS / INSERM / Ecole Polytechnique, France

2008-2009: Post-doctoral position at Nottingham University, UK, in Dr. Thosten Allers laboratory

2004-2008: PhD on replication fork restart in E. coli at the CGM, CNRS, Gif-sur-yvette, supervised by Dr. Bénédicte Michel

 

Enseignement / Teaching


TD (Tutorial class) "Biologie moléculaire et information génétique" (BIO452) for 2nd year student at Ecole polyetchnique 

MODAL (Practical Course) for 2nd year student at Ecole polyetchnique untitled « Bioluminescence et GFP » 

TREX (Practical Course) for 3rd year student at Ecole polyetchnique untitled « Rôle des flagellines dans la motilité chez l’archée Haloferax volcanii » 

Master 2 Biologie Santé, Parcours Microbiologie Fondamentale – Paris XI/Paris Saclay « Une révolution récente en (micro)biologie : les archées »

Licence 3 Sciences de la vie - Université Versailles-Saint Quentin « Réplication et Conversion génique chez les archées »

 

Publications (ORCID ID: 0000-0001-7377-4734)


Sodolescu A., Dian C., Terradot L., Bouzhir-Sima L., Lestini R., Myllykallio H., Skouloubris S., Liebl U. (2018). Structural and functional insight into serine hydroxymethyltransferase from Helicobacter pylori. PloS one, 13(12), e0208850. DOI

Delpech F., Collien Y., Mahou P., Beaurepaire E., Myllykallio M., Lestini R. Snapshots of archaeal DNA replication and repair in living cells using super-resolution imaging, Nucleic Acids Research, DOI

van Tran N., Muller L., Ross R. L., Lestini R., Létoquart J., Ulryck N., Limbach P. A., de Crécy-Lagard V., Cianférani S., Graille M. (2018). Evolutionary insights into Trm112-methyltransferase holoenzymes involved in translation between archaea and eukaryotes. Nucleic acids research, 46(16), 8483-8499, DOI

Sakina K., Lestini R., Myllykallio H. and Houali K. Isolation and identifcation of two extremely halophilic archaea from sebkhas in the Algerian Sahara (2018) Cellular and molecular biology 64(4):83-91

Plateau, P., Saveanu, C., Lestini, R., Dauplais, M., Decourty, L., Jacquier, A., Blanquet, S. and Lazard, M. (2017) Exposure to selenomethionine causes selenocysteine misincorporation and protein aggregation in Saccharomyces cerevisiae. Sci Rep, 7, 44761, DOI

Becker, H.F., Heliou, A., Djaout, K., Lestini, R., Regnier, M. and Myllykallio, H. (2017) High-throughput sequencing reveals circular substrates for an archaeal RNA ligase. RNA Biol, 1-11. DOI

Lestini, R., Delpech, F. and Myllykallio, H. (2015) DNA replication restart and cellular dynamics of Hef helicase/nuclease protein in Haloferax volcanii. Biochimie, 118, 254-263. DOI

Lestini, R., Delpech, F. and Myllykallio, H. (2015) DNA Replication Restart in Archaea, Advances in DNA Repair, Prof. Clark Chen (Ed.), InTech, DOI

Rezgui, R., Lestini, R., Kuhn, J., Fave, X., McLeod, L., Myllykallio, H., Alexandrou, A. and Bouzigues, C. (2014) Differential interaction kinetics of a bipolar structure-specific endonuclease with DNA flaps revealed by single-molecule imaging. PLoS One, 9, e113493. DOI

Lestini, R., Laptenok, S.P., Kuhn, J., Hink, M.A., Schanne-Klein, M.C., Liebl, U. and Myllykallio, H. (2013) Intracellular dynamics of archaeal FANCM homologue Hef in response to halted DNA replication. Nucleic Acids Res, 41, 10358-10370. DOI

Creze, C., Ligabue, A., Laurent, S., Lestini, R., Laptenok, S.P., Khun, J., Vos, M.H., Czjzek, M., Myllykallio, H. and Flament, D. (2012) Modulation of the Pyrococcus abyssi NucS endonuclease activity by replication clamp at functional and structural levels. J Biol Chem, 287, 15648-15660. DOI

Lestini, R., Creze, C., Flament, D., Myllykallio, H., and Henneke, G., (2011). Archaeal DNA Repair Nucleases, DNA Repair - On the Pathways to Fixing DNA Damage and Errors, Francesca Storici (Ed.), InTech, DOI

Creze, C., Lestini, R., Kuhn, J., Ligabue, A., Becker, H.F., Czjzek, M., Flament, D. and Myllykallio, H. (2011) Structure and function of a novel endonuclease acting on branched DNA substrates. Biochem Soc Trans, 39, 145-149. DOI

Lestini, R., Duan, Z. and Allers, T. (2010) The archaeal Xpf/Mus81/FANCM homolog Hef and the Holliday junction resolvase Hjc define alternative pathways that are essential for cell viability in Haloferax volcanii. DNA Repair (Amst), 9, 994-1002. DOI

Baharoglu, Z.*, Lestini, R.*, Duigou, S. and Michel, B. (2010) RNA polymerase mutations that facilitate replication progression in the rep uvrD recF mutant lacking two accessory replicative helicases. Mol Microbiol, 77, 324-336 *co-premier auteur DOI

Lestini, R. and Michel, B. (2008) UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR in a rep mutant of Escherichia coli. J Bacteriol, 190, 5995-6001. DOI

Centore, R.C., Lestini, R. and Sandler, S.J. (2008) XthA (Exonuclease III) regulates loading of RecA onto DNA substrates in log phase Escherichia coli cells. Mol Microbiol, 67, 88-101. DOI

Michel, B., Boubakri, H., Baharoglu, Z., LeMasson, M. and Lestini, R. (2007) Recombination proteins and rescue of arrested replication forks. DNA Repair (Amst), 6, 967-980. DOI

Michel, B., Baharoglu, Z., and Lestini, R. (2007) Genetics of recombination in the model bacteriun Escherichia coli. Topics in Current Genetics, Aguilera A. and Rothstein R. eds, Springer.

Lestini, R. and Michel, B. (2007) UvrD controls the access of recombination proteins to blocked replication forks. EMBO J, 26, 3804-3814. DOI

Janniere, L., Canceill, D., Suski, C., Kanga, S., Dalmais, B., Lestini, R., Monnier, A.F., Chapuis, J., Bolotin, A., Titok, M. et al. (2007) Genetic evidence for a link between glycolysis and DNA replication. PLoS One, 2, e447. DOI

Bidnenko V., Lestini R., Michel B.The Escherichia coli UvrD helicase is essential for Tus removal during recombination-dependent replication restart from Ter sites.Mol Microbiol. 2006 Oct;62(2):382-96.DOI