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SIFT: Label-Free Sorting by Droplet Microfluidics

November 25, 2021, 11AM

Paul ABBYAD, Santa Clara University, USA

Lieu(x) :      Amphithéâtre Pierre Curie, Ecole Polytechnique

Contact :    Antigoni Alexandrou
                    antigoni.alexandrou at

Our lab has developed a novel platform for droplet sorting that can be used to sort enzymes by activity, and cells by metabolism. It is a passive technique that utilizes no excitation laser, detector, active sorter and related electronics, or fluorescent labels. It presents a robust way to sort enzyme libraries for screening and sort cancer cells by a defining characteristic, their aberrant metabolism. The technique also enables the isolation of activated T-cells prior to the display of cellular surface markers. The isolation of activated T-cells in minutes rather than hours can have broad usage as a biotechnology tool and for the study and selection for immunology and immunotherapy.

Dobson, C; Zielke, C; Pan, C;  Feit, C; Abbyad, P (2020) Method for Passive Droplet Sorting after Photo-Tagging Micromachines 11(11), 964.

Zielke, C; Pan, C Gutierrez Ramirez, A; Feit, C; Dobson, C; Davidson, C; Sandel, B; Abbyad, P (2020) Microfluidic Platform for the Isolation of Cancer-Cell Subpopulations based on Single-Cell Glycolysis Analytical Chemistry  92, 10, 6949–6957.

Horvath, DG; Braza, S; Moore, T; Pan, CW; Zhuc, L; Pak, O; Abbyad, P (2019) Sorting by interfacial tension (SIFT): Label-free enzyme sorting using droplet microfluidics Analytica Chimica Acta, 1089, 108-114. DOI: 10.1016/j.aca.2019.08.025

Pan, CW; Horvath, DG; Braza, S; Moore, T; Lynch, A; Feit C; Abbyad P (2019) Sorting by interfacial tension (SIFT): label-free selection of live cells based on single-cell metabolism Lab on a Chip,  19, 1344-1351  Front Cover of Journal; DOI: 10.1039/C8LC01328D


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